How much blood is needed for IVF?) literally, how many milliliters? After submitting blood for genetic analysis I decided to count.
Here is the list of necessary blood tests a woman needs to undergo before entering the stimulation protocol and the test tubes in which they are stored.
✔ Blood type and rhesus factor – red 4ml test tube with coagulation activators to examine serum. These tests are valid for life, so if you have them on hand, you do not need to repeat them.
✔ Clinical general blood test – light lavender 3ml test tube with coagulation inhibitors EDTA to examine whole blood, the number of different cell types, the test is valid for 3 months.
✔ Coagulogram to assess the hemostasis system, or just how your blood is clotting – a blue tube of 2.7 ml with sodium citrate (3 months). By the way, sodium citrate is a salt that is added to the donor blood, such blood will not coagulate and will remain as unchanged as possible, the citrate is then easily excreted by the kidneys of the person to whom the blood will be infused.
✔ Antibodies to HIV, hepatitis C and B, syphilis – one red test tube (3 months)
✔ Antibodies to TORCH infections, infections that have a vertical transmission from mother to child during pregnancy; it’s generally a basic analysis when planning a pregnancy (toxoplasma, cytomegalovirus, herpes viruses 1 and 2, Epstein-Barr virus, rubella, sometimes advised to check and antibodies to chlamydia ) – yellow test tube 8.5 ml with coagulation activator and acrylic gel for centrifugation (6 months)
✔ Hormones: Antimullerian (AMH), prolactin, follicle-stimulating (FSH), luteinizing (LH), progesterone, estradiol, thyrotropin (TSH), triiodothyronine (T3), thyroxine (T4), antibodies to thyroglobulin and thyroperoxidase, antimicrosomal antibodies – 2 yellow test tubes. (6 months)
✔ Biochemical blood test, liver and kidney tests, total bilirubin, protein fractions – yellow tube (6 months)
✔ Glucose – 2 ml or 4 ml gray tube containing EDTA and sodium fluoride, which stops glucose cells from consuming glucose, and sometimes an additional level of insulin (yellow tube) is required to determine the HOMA index and test for insulin resistance. Taken on an empty stomach, valid for 6 months.
And sometimes glycosylated hemoglobin for the same purpose – then a purple test tube with EDTA 4ml
✔ Optional for the first IVF is a karyotype – a green test tube with heparin. And optional molecular genetic testing for carriers of hereditary mutations – a purple test tube, these tests are valid for life.
It may be impossible to test all these indicators at once, because the level of FSH should be checked at the beginning of the menstrual cycle, during the first days. Some indicators should be checked after eating, some only on an empty stomach. Some tests will be added during the program, for example on the day of the puncture an additional test for progesterone may be prescribed. It is the doctor’s decision.
Well, I have counted 8 to 12 test tubes and the volume of blood of about 50 ml))) If a woman loses 50 ml of blood, she will not feel bad or lose consciousness, it is not much. But some people are afraid of blood and may feel unwell because of anxiety. Therefore, in the manipulation room we have comfortable chairs for blood sampling, and nurses are friendly to make you as comfortable as possible!
For the first reproductive specialist’s consultation, it is good to have results of FSH and AMH levels, and a spermogram for men. All other tests will be prescribed by the doctor; you will be instructed how and when they will be taken, but here you have an approximate list.
In the photo you can see the nurse’s work shelf before the start of the working day and after 4 hours of work))